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Cannabis Education

Scientists Create New Method to Store Marijuana Pollen on a Long-Term Basis

Researchers have developed a way to determine the viability of pollen germination in marijuana plants, as well as a simple method of storing cannabis pollen for long periods of time, according to a new study.

For marijuana cultivators, the results could prove useful, helping them avoid potentially costly mistakes in the process and ensuring consistency and quality in their crops across growing cycles.

Ensuring long-term storage of pollen is important for cultivators because of the central role it plays in generating seeds. Taking pollen from a male plant and rubbing it on the hairs of a female plant—typically about halfway through the flowering cycle—will enable the female plant to produce buds that contains seeds, which along with cloning is one way to propagate and maintain strains.

The study, which was partly funded by the Canadian government, has “several implications,” co-author Igor Kovalchuk told Marijuana Moment.

First, the team created an “assay to test viability of such pollen before the use for pollination.”

They accomplished that by modifying an existing method of assessing germination viability, using a liquid media instead of a solid medium, which “resulted in better image acquisition and quantification of germination,” according to the study.

Perhaps even more consequential for growers, however, is the development of a long-term storage system for cannabis pollen.

“We have provided an easy protocol for cryopreservation using desiccation combined with baked wheat flour and subsequent long-term storage of cannabis pollen in liquid nitrogen.”

“This one is big,” Kovalchuk said. “Our protocol allows nearly indefinite storage,” which is “valuable for maintaining large collection of genetics.”

To preserve the pollen, the researchers removed any moisture, added the result to baked whole wheat flour and preserving agents and then froze it in liquid nitrogen. When they removed the mixture from the liquid nitrogen and applied it to flowering female plants, it resulted in successful seed formation in all of the subjects.

The team also discovered that pollen can be more or less viable at different stages of the flowering period. The optimal time to extract pollen seems to be during the mid-flowering stage. At that point, it retained “viability the longest with 22 percent of pollen grains successfully germinating after 21 days” of storage in a low-temperature environment.

“In conclusion, we have standardized a simple assay for quickly assessing pollen germination in Cannabis sativa,” the study states. “By using our [modified assay], we have demonstrated the loss of pollen viability over time when stored at 4 degrees Celsius, and suggested an optimal time during flower development for pollen collection to maximize longevity during storage.”

“Finally, we have provided an easy protocol for cryopreservation using desiccation combined with baked wheat flour and subsequent long-term storage of cannabis pollen in liquid nitrogen,” it concludes.

Featured image by Eric Limon/Shutterstock


This article has been republished from Marijuana Moment under a content-sharing agreement. Read the original article here.



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Cannabis Education Videos

AURORA CANNABIS OPEN FLAGSHIP RETAIL STORE AT WEST EDMONTON MALL



AURORA CANNABIS OPEN FLAGSHIP RETAIL STORE AT WEST EDMONTON MALL – RICH TV LIVE – NOVEMBER 27 – 11,000 Square Foot Retail Destination and Global Centre for Cannabis Education, Discovery and Community Engagement

TSX | NYSE: ACB

Aurora Cannabis Inc. (“Aurora”) (NYSE | TSX: ACB), the Canadian company defining the future of cannabis worldwide, today announced the grand opening of its flagship retail store at 10:00 a.m. MT on November 27th in the West Edmonton Mall, North America’s largest mall. At approximately 11,000 square feet, Aurora has combined both a retail cannabis store and an immersive experiential space, encouraging visitors from around the world to explore unique products and participate in a rotating calendar of programming and events. Aurora’s flagship store offers visitors a safe, age-gated retail experience in full compliance with all relevant federal and provincial regulations.

“Aurora is proud to call Edmonton home. It’s here where we established our roots and built our business. There’s no better place for us to open the doors to our flagship store and to welcome consumers from all over the world to join us in celebrating how far the cannabis movement has come and how quickly it continues to grow,” said Terry Booth, CEO of Aurora. “This space is a reflection of Aurora’s core values, a celebration of our past, and cements our position as an innovative industry leader. We invite new and experienced consumers alike to come learn and explore.”

Following many firsts for the West Edmonton Mall, this partnership makes Aurora the first cannabis retailer to operate within a mall of this size, with Aurora serving as the Mall’s exclusive cannabis retailer. “West Edmonton Mall prides itself on delivering unique, one-of-a-kind experiences to its 30 million annual visitors. We are, in fact, the internationally recognized pioneers of merging retail with entertainment and maximizing the synergies between the two. We often accomplish our goal with world-class partners. What makes this collaboration particularly special is that our world-class and best-in-class partner, Aurora, is based right here in Edmonton. It is only through these unique circumstances that such an incredible project could come together,” said David Ghermezian, CEO of West Edmonton Mall.

To design an innovative retail experience, Aurora enlisted Bruce Mau Design in collaboration with GMC Architects, creating a place for consumers to gather. Inspired by technology, innovation and nature, the space explores the relationship between the natural elements that are core to the Aurora brand and the state-of-the-art production and commitment to industry advancement that drives Aurora’s continued growth. References to mountains, sky, water and sun permeate through material, lighting and digital experiences.

The flagship store will host world-class researchers, creators and culture makers to lead sessions with artists, chefs, local innovators and experts in the emerging world of cannabis to deliver next-level inspirational experiences. In the age-gated cannabis store, visitors will have access to over 42 different cannabis products from the Aurora family of brands, such as San Rafael ’71, Alta Vie, Whistler Cannabis Co and Woodstock, all while being served by passionate and knowledgeable retail staff.
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The Magical Butter Machine @ The Cannabis Super Store



The Magical Butter machine is the world’s first counter top Botanical Extractor™, designed for creating fantastic recipes, infusing the essence of healthy herbs into butter, oil, grain alcohol, lotions, and more!

The Magical Butter machine is scientifically designed for a specific purpose: creating incredible recipes and botanical infusions with little or no labor. The Magical Butter machine combines an immersion blender with a digital thermostat and heating unit. The MB grinds, heats, stirs, and steeps your herbal extract, all at the correct time intervals and temperature. So, you achieve your desired infusion easily, safely, and consistently.

It sounds simple.
Can you use it even if I’m a stranger to the kitchen?
Absolutely! They replaced all the tedious guesswork with predictable results and a cool light show. Just add your ingredients, press two buttons, and get ready to…Eat to Treat

For more information follow us on:

Facebook : https://www.facebook.com/TheCanabisSuperstore
Instagram: https://www.instagram.com/cannabis.superstore
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Email: info@thecannabissuperstore.ca
WebSite: https://thecannabissuperstore.ca
Call: (416)894-8105

OR come visit us @

2775 Lake Shore Blvd. West
Toronto, Ontario

We specialize in Cannabis Education, Premium Gear, Growing Equipment, Extraction Equipment, Genetics, Federal Compliance, Health and Safety, Access to LP Products
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#hydroponics

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Legal Cannabis Haul from BC Cannabis Store on Cannabis Legalization



I went and shopped in BC Canada’s first legal cannabis store. I purchased seven different cannabis products including cannabis flower, oil drops, CBD capsules and three different types of pre-rolls. Overall I was pleasantly surprised by this government owned and operated cannabis store.

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We believe that, ultimately, safe consumption education is the best form of harm reduction.

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User-Generated Video by Bethany Rae

Disclosure: The information shared in this video includes personal experiences and is not to be considered medical or legal advice. Please consult with a health practitioner and seek legal counsel on the cannabis laws in your area

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Cannabis Training Videos

Cannabis: Dollar Store Low Stress Training



Cannabis: Dollar Store Low Stress Training
Rise Up Motivational medicine and trichome cultivation Bud Porn Inspiration

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Legalize and cure the World… Respect!

*Medical Grow Prop 215 sb420

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