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Cannabis Education

Scientists Create New Method to Store Marijuana Pollen on a Long-Term Basis

Researchers have developed a way to determine the viability of pollen germination in marijuana plants, as well as a simple method of storing cannabis pollen for long periods of time, according to a new study.

For marijuana cultivators, the results could prove useful, helping them avoid potentially costly mistakes in the process and ensuring consistency and quality in their crops across growing cycles.

Ensuring long-term storage of pollen is important for cultivators because of the central role it plays in generating seeds. Taking pollen from a male plant and rubbing it on the hairs of a female plant—typically about halfway through the flowering cycle—will enable the female plant to produce buds that contains seeds, which along with cloning is one way to propagate and maintain strains.

The study, which was partly funded by the Canadian government, has “several implications,” co-author Igor Kovalchuk told Marijuana Moment.

First, the team created an “assay to test viability of such pollen before the use for pollination.”

They accomplished that by modifying an existing method of assessing germination viability, using a liquid media instead of a solid medium, which “resulted in better image acquisition and quantification of germination,” according to the study.

Perhaps even more consequential for growers, however, is the development of a long-term storage system for cannabis pollen.

“We have provided an easy protocol for cryopreservation using desiccation combined with baked wheat flour and subsequent long-term storage of cannabis pollen in liquid nitrogen.”

“This one is big,” Kovalchuk said. “Our protocol allows nearly indefinite storage,” which is “valuable for maintaining large collection of genetics.”

To preserve the pollen, the researchers removed any moisture, added the result to baked whole wheat flour and preserving agents and then froze it in liquid nitrogen. When they removed the mixture from the liquid nitrogen and applied it to flowering female plants, it resulted in successful seed formation in all of the subjects.

The team also discovered that pollen can be more or less viable at different stages of the flowering period. The optimal time to extract pollen seems to be during the mid-flowering stage. At that point, it retained “viability the longest with 22 percent of pollen grains successfully germinating after 21 days” of storage in a low-temperature environment.

“In conclusion, we have standardized a simple assay for quickly assessing pollen germination in Cannabis sativa,” the study states. “By using our [modified assay], we have demonstrated the loss of pollen viability over time when stored at 4 degrees Celsius, and suggested an optimal time during flower development for pollen collection to maximize longevity during storage.”

“Finally, we have provided an easy protocol for cryopreservation using desiccation combined with baked wheat flour and subsequent long-term storage of cannabis pollen in liquid nitrogen,” it concludes.

Featured image by Eric Limon/Shutterstock


This article has been republished from Marijuana Moment under a content-sharing agreement. Read the original article here.



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Cannabis Training Videos

Cannabis your/ my method of training(leaf building)for “bigger buds” experiment

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Cannabis Training Videos

Sea Of Green Method



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Time Stamp for Video:
(00:09) : What is Sea of Green
(01:17) : Pros of Sea of Green
(01:30) : Cons of Sea of Green
(02:22) : Making your own Sea of Green

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Cannabis Super-Cropping: Kyle Kushman's "Chiropractic" Plant Training Method / Green Flower



Introducing his famous “chiropractic” technique, award-winning cannabis cultivator and breeder Kyle Kushman demonstrates the proper way to pinch, prune and “crack” your cannabis plants’ branches, nodes and stems to increase canopy and yield.

Also in this comprehensive look at how to “supercrop,” trim and train your cannabis, Kyle will show you step-by-step, what to clip, how much and when.

But wait… there’s more! Don’t throw away your trimmings. Kyle will show you what to do with the leftover cannabis clippings and leaves, from creating clones to juicing.

To learn even more in-depth expert cannabis cultivation tips and techniques, directly from respected names like Kyle Kushman, Kevin Jodrey, Mel Frank and more, check out Green Flower’s latest Cannabis Cultivation Certificate Course, now open for a limited time at https://green-flower.com/academy/cultivation

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Dont Grow Blind Use Method Seven Eyewear- The Cannabis Superstore



Method seven technology blends incredible color balancing for each of the standard grow lighting spectrum’s (hps, metal halide, led, sun) with exceptionally high-quality lenses that provide the best in optical clarity.

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LED GROW -The world’s first glasses formulated to light balance and compensate for the unique spectrum of LED grow lights. Our M7 lens technology, highest quality materials, and manufacturing by Carl Zeiss Vision provide exceptional color and clarity.

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How To Make Cannabutter – Easy Cannabutter Method For One Stick of Cannabis Butter



While it may feel much more intense than smoking, edible cannabis is one of the safest ways many patients choose to consume their beloved medicine.

Cannabutter has become one of the most popular infusions for many cannabis lovers across the world. Learn how to make an easy cannabutter that you can add to an incredibly wide variety of dishes.

How To Decarb Cannabis: https://www.youtube.com/watch?v=ijJFJZZ5-ts

Full Recipe: https://cloudykitchen.co/easy-cannabutter-recipe-how-to-make-one-stick-of-cannabutter/

This easy method involves the stovetop, however, if you want to use a slow cooker, check out our other method right here:

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For all of our other cannabis recipes: https://cloudykitchen.co/category/cannabis-lifestyle/cannabis-recipes-and-infusions/

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Training Sativas In Flower 3 Weeks Into Bloom HST Training Method



Training the Sativa’s in week 3 of Bloom using high stress traing method or HST. Growing Serious Six form Serious Seeds & Cindy 99. Also did a quick foliar spray of Optic Foliar Overgrow

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Cannabis Training Videos

Soil Recycling & The Probiotic Grow Method – Cannabis Training University



Cannabis expert Dustin breaks down the cost benefits of using recycled soil and a probiotic growth method to grow your cannabis plants, as well as the challenges faced during the drying process. Enroll Now: thectu.com/enroll-now

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how to grow short weed plants with lots of tops lst training new lst training method step by step



how to grow short weed plants with lots of tops lst training.this is how i lst the Apical meristem of a plant.new lst training method step by step.

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Cannabis Training Videos

How To FIM (Fuck-I-Missed) a Cannabis Plant – Stress Training Method + Grow Update 05/08/2017



A quick FIM stress technique method to improve yield and distribute growth into multiple shoots while still maintaining the main stem instead of topping in which the main stems splits into two branches and grows off from there. Any questions or comments? Post below! Be sure to comment, rate and SUBSCRIBE! Thanks!

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