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Cannabis Education

Scientists Create New Method to Store Marijuana Pollen on a Long-Term Basis

Researchers have developed a way to determine the viability of pollen germination in marijuana plants, as well as a simple method of storing cannabis pollen for long periods of time, according to a new study.

For marijuana cultivators, the results could prove useful, helping them avoid potentially costly mistakes in the process and ensuring consistency and quality in their crops across growing cycles.

Ensuring long-term storage of pollen is important for cultivators because of the central role it plays in generating seeds. Taking pollen from a male plant and rubbing it on the hairs of a female plant—typically about halfway through the flowering cycle—will enable the female plant to produce buds that contains seeds, which along with cloning is one way to propagate and maintain strains.

The study, which was partly funded by the Canadian government, has “several implications,” co-author Igor Kovalchuk told Marijuana Moment.

First, the team created an “assay to test viability of such pollen before the use for pollination.”

They accomplished that by modifying an existing method of assessing germination viability, using a liquid media instead of a solid medium, which “resulted in better image acquisition and quantification of germination,” according to the study.

Perhaps even more consequential for growers, however, is the development of a long-term storage system for cannabis pollen.

“We have provided an easy protocol for cryopreservation using desiccation combined with baked wheat flour and subsequent long-term storage of cannabis pollen in liquid nitrogen.”

“This one is big,” Kovalchuk said. “Our protocol allows nearly indefinite storage,” which is “valuable for maintaining large collection of genetics.”

To preserve the pollen, the researchers removed any moisture, added the result to baked whole wheat flour and preserving agents and then froze it in liquid nitrogen. When they removed the mixture from the liquid nitrogen and applied it to flowering female plants, it resulted in successful seed formation in all of the subjects.

The team also discovered that pollen can be more or less viable at different stages of the flowering period. The optimal time to extract pollen seems to be during the mid-flowering stage. At that point, it retained “viability the longest with 22 percent of pollen grains successfully germinating after 21 days” of storage in a low-temperature environment.

“In conclusion, we have standardized a simple assay for quickly assessing pollen germination in Cannabis sativa,” the study states. “By using our [modified assay], we have demonstrated the loss of pollen viability over time when stored at 4 degrees Celsius, and suggested an optimal time during flower development for pollen collection to maximize longevity during storage.”

“Finally, we have provided an easy protocol for cryopreservation using desiccation combined with baked wheat flour and subsequent long-term storage of cannabis pollen in liquid nitrogen,” it concludes.

Featured image by Eric Limon/Shutterstock


This article has been republished from Marijuana Moment under a content-sharing agreement. Read the original article here.



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TRAINING YOUR CANNABIS TEAM w/ MJ STAPLEY – Weed To Know Basis Episode 31



TRAINING YOUR CANNABIS TEAMS w/ MJ STAPLEY – Weed To Know Basis Episode 31 is out NOW! In this episode we talk with Melissa “MJ” Stapley, the founder and CEO of MJ Hybrid Solutions.
MJ Hybrid Solutions is an online sales training and cannabis education program for dispensary teams, product companies, and all sales professionals in the cannabis space. After discovering a need for cannabis employee training, our CEO MJ Stapley took her experience and passion for sales training and cannabis education to develop the MJ Hybrid Training System. The MJ Hybrid Training System is a two-fold approach training for cannabis employees that is both scientifically accurate and helps employees better sell to customers. The cannabis education is written and backed by medical cannabis doctors and scientists with over 15 years’ experience and our sales training is a proven methodology to help companies increase business profitability, customer retention, and employee engagement. Our mission is to legitimize the cannabis industry through proper employee education and training.

Sales Training
The cannabis industry is growing at a fast pace. With new products and new customers it is important to train your staff on effective sales strategies and customer service.
Cannabis Education
It’s critical that employees understand what it is they are selling. With so many different products and ratios, it is not only important to know who they are selling to, but what product might be best for each individual customer’s needs.

FOR MORE INFORMATION PLEASE VISIT:
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INTRO MUSIC BY:
Rock Angel by Joakim Karud https://soundcloud.com/joakimkarud
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